Journal: npj Imaging

Article Title: Quantitative multi-metabolite imaging of Parkinson’s disease using AI boosted molecular MRI

doi: 10.1038/s44303-025-00130-x

Figure Lengend Snippet: The white dashed lines in all panels mark the cortex-striatum boundary. a Glutamate (Glu) and DAPI staining. The white arrows highlight Glu-positive signals in the striatum, presenting an increase after MPTP administration. b Glu receptor (mGluR3) and DAPI staining. c Glu and mGluR3 receptor staining. The yellow arrows indicate double-stained areas in the striatum, demonstrating enhanced expression in the MPTP-treated mouse. d GFAP and DAPI staining, with an increased GFAP signal in the MPTP group, reflecting higher astrocytic activation (with DAPI providing background cell body visualization). e A merged image combining the signals from ( a , b , d ). f Coomassie blue staining. The increased staining intensity in the MPTP-treated brain suggests an increase in total protein content.

Article Snippet: Antibodies against glutamate (#AB5018, Sigma Aldrich), GFAP (#Ab4674, Abcam), and mGluR3 glutamate receptor (#AGC-010-GP, Alomone labs) were used for IHC tissue staining.

Techniques: Staining, Expressing, Activation Assay